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CRIMIC CR70843

Gene Orc3

CG4088

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:13202728..13206547	49F7-49F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAGCGTGCTGCAGGATC	CGCCATCGAGACTCTGAAAC

Images

Construct Design Sequences

sgRNA sequence	GAACTTTATCCAGCACCTAAAGG
sgRNA2 sequence	GAAAAGATAAAAATACCTAAGGG

 

left_200bp	right_200bp
CTGCGGCTCGCTTCCATTCGTCCTGGTCCTGGGAGTGGCCACGGCCATGACAGCTGTCCATGGGACACTACCATACCACGTCAGCAGCAAGATTCGTCTAAGGGTGTTCCAGACTCAAGCTGCTCCCACGGGCCTTAATGAGGTAATATTCCTTAACTCTGTGCTAGTTTATGTACTAATCGGTTTTCTAACAACCTTTA	GGTATTTTTATCTTTTCTATTTATTTATATTGGGGCAAATTTCATGTGTGGGACGTTATGAATAAATAAAACATCAATCGATTTGTCTACTCTGTACGAAATGCATTAACTTTGTTTGCGAGTGAAGCGCGTTCTTGTATTTTTTAAGTAAAGCCCAGCAGCTGGCAAGGCAAGCGTGCTAAATATCAATTACCAGTGTT

 

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