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CRIMIC CR70844

Gene Eglp3

CG17662

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:23605689..23606654	59F4-59F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGGCACACAAGAAGTACGAGTA	CATTTCATTCGCTATCAAGCTGAGC

Images

Construct Design Sequences

sgRNA sequence	CTTCTGTTGCGACATGCTGACGG
sgRNA2 sequence	CGGAGAGGTTATAGTGCAATAGG

 

left_200bp	right_200bp
TTACGGCTGAGCAAAGCCCCCCGAAAATCGCCTTGATGGATTGATCCATCCTGAATGGGTGGGCGGGAGCTGCTCCCAAATCTGCGCTCGATAATTAGCATTAAGGTTTTATAATGGATTTTGATTGATTGCCGTAAATTTTGCTGGCACGTCGGGTCACTTGGGCAGCCGCCCAAAATTGCGGCAGTAGAGTTCCGTCA	AATAGGTGTGAATTGAATCTTATCGAATAAACTATATATATATACGAATATGTTGTAATAATAGAGCCACAAAATATTTAGGTCGATTAAGTAATTAAGCACCACCTTGTCTTATTTCCATAGCATACAATTGGGATGAGCGGCGATATTGGCCATTTTTTTGGGGGCTCGTATTCGCTGGAAAGTTTGACGAGTTTGTC

 

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