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CRIMIC CR70845

Gene CG18278

CG18278

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:13408042..13409887	50A5-50A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTACTGTTGTCTGGCAACGC	CTCACGCATTTAACCAGCGC

Images

Construct Design Sequences

sgRNA sequence	ACAACACTGCACTTGCAAGCCGG
sgRNA2 sequence	ATATCTATACAAAACACGAGTGG

 

left_200bp	right_200bp
TGGAAGAATATTTGAATTTGAATAATTCGCGCGCCTGAGAGCTGATGTTCGTATTTTCACAAGTGGTAAGTGCAGTTTACTAAATAGGTAAAAATGATACTTTCAATATAATTTGATTAGGTTTAGAGTTTGTCCTATTTCGGTTGGCCATTGGCTAACACTAAAAGATCGCCGCTGCCTTTTACAACACTGCACTTGCA	GTGTTTTGTATAGATATATGCATATTACTCCTAGCCAAATAGTAATCCAAAACCAAATTCAAGTCCGCTGGGCGCATATAGATTTCATATTCAGATATCATGTGATTTATGTTGCTATATACATAGTCTCATATTAATCGTATTTTACAGTCTAAAGTTACACTTATTTGAAACCGTTTCAGTACTGAAAACATAAATAA

 

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