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CRIMIC CR70846

Gene CG30059

CG30059

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:13412769..13414620	50A5-50A5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTACTGTTGTCTGGCAACGC	CGCTCAGCACGTAAGCAATC

Images

Construct Design Sequences

sgRNA sequence	ACAACACTGCACTTGCAAGCCGG
sgRNA2 sequence	ATATCTATACAAAACACGAGTGG

 

left_200bp	right_200bp
CTGGAAGAATATTTGAATTTGAATAATTCGCGCGCCTGAGAGCTGATGCTCGAATTTTCACTGGTGGTAAGTGCAGTTTACTAAATAGGTAAAAACTATATATTCAATATAATTTGATTAGGTTTAGAGTTTGTCCTATTTCGGTTGGCCATTGGCTAACACTAAATGATCGCCGCTGTCTTTACAACACTGCACTTGCA	GTGTTTTGTATAGATATATGCATATTACTCCTAGCCAAATAGTAATCCAAAACCAAATTCAAGTCCGCTGGGCGCATATAGATTTCATATTCAGATATCATGTGATTTATGTTGCTATATACATAGTCTCATATTAATCGTATTTTACAGTCTAAAGTTACACTTATTTGAAACCGTTTCAGTACTGAAAACATAAATAA

 

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