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CRIMIC CR70848

Gene Sulf1

CG6725

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:16310542..16339575	89B12-89B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCCAAGTACTACAACTACAGCATCAAC	TTGTGAACAGGCTGCATGGG

Images

Construct Design Sequences

sgRNA sequence	TTCGTTAATAATTAAGTAACAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGCCTTCCTCCGCTCCTCAAAGCAACAGAACCAGCGGAAGCCAGTGCTGCTCACCATGAGTTTTCCTGCACCACATGGCCCTGAGGATTCGGCTCCCCAGTATAGTCATCTCTTCTTTAATGTGACAACCCATCAGTAAGTAAATACCCTAACTATTAACACCCGATAATAGTTGAACTTAATTCGTTAATAATTAAGT	AACAGGTTGAAACGATCCTTGTTCCTTTATTTATTTCGGTTTTGCAAGTTTCTTTCTTTAAATATGCAAACAGTCTGTATGATTAAGAATATCGATTATATTGTTCTTTCTTTTTGTCAATAGCTTTTAACATTTAATTTGCACTTATAGCACTCCATCGTATGATCACGCCCCAAATCCGGACAAGCAATGGATCCTGA

 

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