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CRIMIC CR70849

Gene Hs6st

CG4451

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:19929277..20008512	92B9-92C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	multi	CCGAAATTTATACCCGCACCTC	GAGACATTTAAGGCACAGCAGG

Images

Construct Design Sequences

sgRNA sequence	AAACTTTGGCATCGGCTTTCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTAGGTTTTTACGGCGCATTTTCCGCATTTATGTATATGTTTTGTCCCCCTTTTTTTTTGGGCTTAGTTTACTTACCAAATTACAGGGGAATGAACAGCATATGAAATTCGTGTATTTATATCACTGTTTTTATGTCTCTATTAAAACTGCACAACACTTGTCGTTACCAGCTAGATCGTGCCAAACTTTGGCATCGGCT	TTCGGGCCATAAAAAGTTTACTCCGTGAATACATTTTGGTTTTTGCTTACGCGTTTTATTAGCAAAGATTTGTTTGTCCGCTTCATTTCATTGTCGTCGTTTTATGGCAGCGTTAATCAATTTAATTGAGTGTGGGATTTCTACATTTTTATTGAGAATTTTTTTGGTAGGGCACTTTATCAGATTTGTCACAAGATTAT

 

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