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CRIMIC CR70850

Gene Meltrin

CG7649

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:14664907..14697292

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATAATGGCAGATGGTTGAGGAAAGA	CATCGAACCCGATCGTCATAC

Images

Construct Design Sequences

sgRNA sequence	TTCTTGCCGCTCTTGAATCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTGATTTTTAGGGTATCACAACTGCAATATGTGAACAGATGTGTCTGGAAAATAACTTAATTATGCATATTTTGAGTGACAGAATAATAAAGTACCATTTTGTCAATTCTAAAACTATTTCTTAGTTAAACTGATGTCTTATCAATAACTAGTTTGAGTTTCACCTACTCCAATCAATATTCTTCTTGCCGCTCTTGAA	TCGAGGTTGTTGCTTAATCAAGTTCAGCTAACCAATCGCACAAGTACAGTGGTTAAAATGGCCTGCAAAGAATGAGTTAAATGTATTTAAAGGAAAATTGATAACAATTTATGCAAGTGTTAGTATCAGCAACGAAATGGGGATATAAGTTAGATATACTATCTGTTCGTGAGTCATTCTGTGATTGGGCTACTATCAAG

 

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