CR70851

CRIMIC CR70851

Gene heph

CG31000

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:31846987..32015520	100D3-100E

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CCATATAACCTCTATACCAAAATCTCCC CACACAAATTTGTAATTATGGATACCTTCC	CTTGGTATGCGTGCGGTTTC

Images

Construct Design Sequences

sgRNA sequence	GAAAGCGTGCCTGTATTTGTGGG
sgRNA2 sequence

left_200bp	right_200bp
GCACATTTTCCGGTTTTAAATGTTATTAATGTGGCAATTAAGATTTAAGCCACAGAAAACAAATGAAGCAGCTACCATTTTATATGCAAATGTGGGTCGGCCCTGCAAACGTTCTGATTAATTACACTTTCTGGGCAACTTAAACCCCGCTCCTTAAGAGATTTGGTCATCATAACATCCTTTGAAAGCGTGCCTGTATT	TGTGGGCGCTCTAAACTGATTTCTAATTTTAATTTCGTAAGCCACAAAGTCACTGACAAAGTTTAAAAAGCTTTCAAATAAATACACATGGCCGCGCCGACATTGGCACACACACGTGTCTGACAAAAGCAGTAAAATGCATAGTTTTTGGGGTTTTGCGGACAGCCCAGCCCCACAGAGAAATTTAGTTGGTGATGTGG