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CRIMIC CR70852

Gene GAA1

CG3033

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:5870841..5873357	5C5-5C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAACACATTTCTTGTAGGTTAGCTT	CAGCGAAGACGGAGGATTGAC

Images

Construct Design Sequences

sgRNA sequence	TTGTGATTCGGGATATATACAGG
sgRNA2 sequence	GGTTTATCACACTTCATCTTAGG

 

left_200bp	right_200bp
TATTTTGATATAATAAAAAATATATATAGAAATATCGAAAACATTGGAAAAACAAGTTTAAAATTTCGAGCATTAAAATCCATTGCTAACCCTCTCCTCCCTAGCGTCATAAGCAATCGGTTATCGAGGTTATCGATAACGCCCGCATCGATACAATTCCGCCGGCTTGTCCGATTCGTGTTGTTGTGATTCGGGATATA	ATGAAGTGTGATAAACCCAACAAAACTGTGTGTGATGGCGGTAAGAGATTGCACATGCAGAGAAAATTCCATTTAAATGTTATGCATTTGATTAAATGTCTAAATAAAAGAAGCCTATTTATTAGTTGGAATAACTAAGTGTAAATGTAATCCAAAAAATATTAAATATACCACGGCATTTTCGATGTTGATTCCCAGTT

 

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