CR70853

CRIMIC CR70853

Gene PIG-O

CG12263

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:18426421..18430333	55C9-55C9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGATGTCTTTTTGATAAATGATGTTTGGG	GTTTGTAGCTTAATCGCAACCG

Images

Construct Design Sequences

sgRNA sequence	AATTGCACTTTACTTCGGAGTGG
sgRNA2 sequence	AATTAATTGGCTGAGACTAGTGG

left_200bp	right_200bp
AGTTGACAACTCTGATTTAACCCAGGGATCAACGTAAAACAGCTGTTTGCTTTGACGAGGTGGCAACGCCATAGAGGGTTCTTGCATACTTCTCACTTTTTTCTTCAGGCCACAAGAAAATCTTTAGATATGAGGAATTTTAAATGAAAACAGCGAGCAAACAGAACGCATCGTGATGCCGGAAACCTAATCAACCACTC	GTCTCAGCCAATTAATTATTTATTTCGGCCTTAATACACAAACAAGTAATAAGAAAATGCAACTGTTTGGTGGTCTTGTAGTTGTAATCTTATTTAGTTAAAATGATTGGGTTCACAAATTTTCCAAAAGACACAAAAACGTCATATGATATCGCGGATCCATGGATTTACATAGTTACTAGTGGGTATGGCTTACAAAA