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CRIMIC CR70858

Gene Snx6

CG8282

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_Kozak	2L:8218071..8280741	28F3-28F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGTCTTGTGTGGTTAAATACACG	GCTGTAGAGAGGAATGCTTGTTTG

Images

Construct Design Sequences

sgRNA sequence	TTTCAACTAGAGGTGGCATCAGG
sgRNA2 sequence	TACTTCCGGCTCCCCGTCTCAGG

 

left_200bp	right_200bp
AAAAATCTAATATAAATTCTCGTGGCAATAAATTATGATATTAATCGAATATTAATGATATTTTGCTGGTAGAAATAAAAAGATTTAATTCCGTAGGAGTGAACCATTATATTCATTTTCGCAATTCAAATTCGATAGCAAGCTTTTTCGATATCGATGTTAGAGCTGCGTGTTATCGATTATTCAGTAGTCCGCCTGAT	ACGGGGAGCCGGAAGTAGACAAGGAATAGAAGGATGAGTCACCCGCACATTGTTTACACACCCCATTGAAACGCAAGCCTCATCGATTGCACACATCCGGGAGACGAAGTAAATCAATTGTTTAATTGCTAAGCAAACAGTTTATTATTGCACAGGCCAAATCGAGTGCAACATTATTATCTTAGCCCACGTATTATATT

 

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