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CRIMIC CR70859

Gene Sas-4

CG10061

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:7151367..7154219	84C6-84C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTATCTTGCAGTACGGCGAGG	CGGAGTTCACATTCGAGCAAATGG

Images

Construct Design Sequences

sgRNA sequence	TGTTTAGACGCCGTACGCGTAGG
sgRNA2 sequence	ACTATGCCAAGTATTAGAGTCGG

 

left_200bp	right_200bp
AAGGACTTTGATGAACCGGAGGAAGGGGATCAGGATTGAAAAAAATCAATTTTTCATATTGCTTAAGAAAATGTACAATATTTTATTTTTAATAAAAAATTAAATCTAATCAAAACGCATGTCTAGCACCATCCCTGACCGCAGAGTTGCCACTGGTACAGTGTTGGACAACTGCTGCCGTAATTTTAAATTCCCCTACGCGTACGGC	AGTCGGGTCTGTGCTTCCGTTGTTAAGTAAATTAACTTAAGTTTTAGATAAATAGAAAAGACGTGCTATTTTTAAAAGATCATCGTAATATTCTTTTCTTGCTGATAAATGGGAAATTGGCTCTATTTTAGATTATTATATATATTGTTTGGTTTTCAATTTGTTAGATCAAATGTAGTAAATTATTCATAGAATGGTAT

 

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