CR70861

CRIMIC CR70861

Gene CG9784

CG9784

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:16595136..16599215	14F2-14F2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTAGTCGATATCGTCATCTTCTC	ATCTAGTGCCATGACACCGTAC

Images

Construct Design Sequences

sgRNA sequence	ATCGAAGAGATTAACCCAACTGG
sgRNA2 sequence	TTAGATCAACACGGCGGTTATGG

left_200bp	right_200bp
TGCTGGGCAAACAAGTGTTAAAAACTATTTGTGCTGCAATATTTTCAGCAATTACCGACCGCCCTCGCCGCTGTTCACCACCCAACTTAACCAGCAACCCAGATTGGAGCAACAAAATCCACAAAGGCATAAAGAATCGACTTACACAATAAAACCTTAATCGGTTGGGTAAACAACTTGATTGCGGTGAGCAGCCAGTT	CCGCCGTGTTGATCTAATTTTATTGACCTCATGTGACCGTGTTAAGTTTGCTTGTTAGCTGTTGACTCAGCGCCTCCTCTCCTCTCCCCCCCTCCAAAATACGTTAATACATACATATGTAATTATACATAATTATCGAACCAATAATATTTGTGTTTACTACCAATTCCACCTATTCCCGCCCTGAATGAATCCCGCTT