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CRIMIC CR70862

Gene Synj

CG6562

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22080176..22085767	58B4-58B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCTCATTTGAGCTATTACGAAGTG	GAGTGGGGTAGTTTGGGCTTTAG

Images

Construct Design Sequences

sgRNA sequence	ACTTAAACTGCAGACAGTAAAGG
sgRNA2 sequence	AGCTGCAGGCTACGCACACGGGG

 

left_200bp	right_200bp
GCATTGCAAACGACGAATCCCAATATTAAACCTACACCTTAAAAACGAAGTACTATTCTTATAAAAATAATAGATCTCTATTATGTAACTGATACACCCTTTAAAAATGTAACTTGTGAAATACACCCACAATTAAATTGTATTTCCCCACCAACAGATGTCTTAGCTTTAGCTGGACGCAAAACTTAAACTGCAGACAG	ACGGGGTTGGTTAAGAATTCAAGAAATATCAAGCGTAATCCGCAACTAATCATATAATGGCTATATTTACAGCTCGCTGTCTTGCACTCCGATTGCATTCCGAATCGCTGTATTTAATGTTATATATACTCGAATATATTGGGCCACTCTGGAGGAGAACTCCTGGCAATATCCGCACTTCGCATGGAATCTATGTTACT

 

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