CR70864

CRIMIC CR70864

Gene CG1894

CG1894

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28484557..28486062	98D2-98D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATAGATCAAGGTAGCAGGGTGTG	CAATGGGCTTGAGGAATCAGTTC

Images

Construct Design Sequences

sgRNA sequence	AAAAGGGTGAAATTCGCAATAGG
sgRNA2 sequence	CTCTCCTGGAAGACACCCACAGG

left_200bp	right_200bp
TGCTCAATATATGTTCAGAAAGTTAAATTGGAATTAAAATTTAAAAATAACTTTAATGCTTTGATATTGGTGCTTCCTTAGATGATTCGATTTTTTGACAACAAACTTTATCGTTTCATTATTGATATCACTAATCGATATCAAGCTAAATGTGTCATCACTAGTGAAAAACCACGCTTTCTAAAAAGGGTGAAATTCGC	CACAGGACGAAAGTAGAACATTTTCATGATATATGTATGCCACTTTGTATGCCAACAATTTACACTAGTAAACTGATTGCACTCATCTACAAGAATGTCCTTATCTTATTTTATTTTTTGCGGTGTACCTTAGACAGTCTAAAATAAAAATCACATTAGCGAGATAATCAAAATTCAAGGCCTGCGGAAATTAGGTAAAA