CR70866

CRIMIC CR70866

Gene mof

CG3025

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:5873840..5876853	5C5-5C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGCGTTAATTCTGTAGGATAGATG	CTATCATTAAGAGTTACAACAGCCTATCC

Images

Construct Design Sequences

sgRNA sequence	AGTGAACAAAAATACGCAATTGG
sgRNA2 sequence	ATATGCGATGATAGCAGAACGGG

left_200bp	right_200bp
GAAGGTTTTGGATAAATAAAATATATTATAATACTGAATGCACAGAAAAATCCAGAAGGGTAATTTCAAAAAGGGTGCAAAGTATTGTACATCGATAACCACCGATTAATAATCGATTATCTGGACATGTTGTGATAAATCAGGGTTGCATTTCACGCCGTATTTCGTTTCATTTCCCTAAAGAGTGAACAAAAATACGC	CTGCTATCATCGCATATAATTGTTATTGACGATGACGATTAGGTTTTTTTTTTTTATTTTTTTTTAAAGAAAGCGTAGGAATAAGCTATTCTATTGCACCCCTCCAAACGAAAAATGTATAGTCATATATCTAAATGGAACCATCAGAAGGTTCAATGTAATACTTAACATTTGGTTTACAATTTTTTTTTTTTATAGTT