CR70867

CRIMIC CR70867

Gene Nepl12

CG8358

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:9675523..9677778	85E3-85E3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTGTTGTTATTCACCCGATATTCAC	ACTTCTGACTGACAGCCTGACTTG

Images

Construct Design Sequences

sgRNA sequence	TTATGGGAACACTTCTCAATTGG
sgRNA2 sequence	AATCTATGATCTACCAAAGTCGG

left_200bp	right_200bp
TAAATACTAAATATCAATAAATATCTCTTATTTGTATACAGATTGTAAACAATAACTTACTCGCTTCCTTATCAACAATCCGTTGCACATTCTACTGATAAGGGTTTTTTATATCTCTCTATTATTGCTCTCTTGATTTTATTGGGATTCTGCCAGTTTTCGTTCGCGATTTAAATCAGTGTGTTTTATAAGTTCCAATT	TTGGTAGATCATAGATTTATAATCATCATCATAAATTCCTTGAACCAAATAAAAGTTGATTTCAATACTTTTTACCGACAATGTAAAATGTTTTGACACAACACTTCAAATTTACGTTACACGTAACAAGCTAACATTTGTGGTTTGAGTACAACTATCGAAGTTTCGCACCCCAGAGCACCGTTCAAATTTAGTCACAT