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CRIMIC CR70868

Gene Nepl20

CG14527

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28880792..28882945	98F1-98F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCACGTTATAGTGTAATAACTGGGTGG	GTTGATTACTCTGCCATGACGTCTAG

Images

Construct Design Sequences

sgRNA sequence	ATTAGCTAAGCTGCCGAAAAAGG
sgRNA2 sequence	AGGGATGAGCACTTCAGTAATGG

 

left_200bp	right_200bp
GCTTTGCATTTTATTATAGTCGATAGCATTACGATTATATACTAGAGAATAGAGTACATTGAATTTAAACGGGTTTCCCTTCTTTGCATGAACTTCGACGATTCTCATTTTTAAATCTATGCTAAACCACGGAGATTTGGTTACCACCTCTAAGTTATAAGAAGAAAGAAAGCGATAAGCCTAAGCGCTCAATTCGTTTTTCGGCAGCTTAGCTAATAAGCTTGGTTCATTAAAATATTTCATTTTAA	TAATGGTTAATATCGGACTTAATTCCATATTTTCTTATTCCATACCTCAACCTTAATTCATAAAGTGATGTTGAACAAATAAAATATAAATTCGCCTTTTTACAATGTAGGACGTGTCAATGTTTTTAGCGAAATGTGGAGATTCTGCTAGCGTCACAATTCGGAAACGTGCTGGTTGGGGCTTATATTGTTTGAGGTTT

 

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