CR70869

CRIMIC CR70869

Gene Nepl7

CG15485

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12667248..12669714	33F2-33F2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGAAGAATGCCAAGATTTGTGCG	CCAGATTTTTGCAACGAACAGAAATCC

Images

Construct Design Sequences

sgRNA sequence	TTAAATTCGAGTTGCGCGCCCGG
sgRNA2 sequence	CGCTCAAGAAGTGCGTGGTTTGG

left_200bp	right_200bp
TAGAACCATAAATTGTTCCACTAATGTGATTTCTGTGGTGAATCCGAGTGTGTTGTATTACCTAATCCACTGAAGCGTGTGCTCAGTGCCCCCCTTTGACCCGAATGCCCACGTTTTTTGGTTTTGGCCCGATCATGATTGTATAAATTGCCAAAGCGCGTTGCCAAAAAAGTTTAGAACTTTTCTGTCTGCTGCCGGGC	GTTTGGTGAAATAAAATCGATTTGTGAAAAATTTTTGGGGCGTTGCTTATAATAGTTGGCTTTAATATTGGGAAATATTTGAAATGCTATCAAGAGATAAATGCATAAAAAGATATAAGAAATTATGTCACTATTTTTATATTTTTAGGATTATCTTGTAACAGGAAATAATACTGCACGAGTAGCCGAAAGTAATGAAG