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CRIMIC CR70871

Gene tj

CG10034

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:19464267..19467758	37E3-37E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAAGAGAGCGAGCTTACAAATG	ACAGTGGTGGTAATGGGAATGC

Images

Construct Design Sequences

sgRNA sequence	AAGGGAGTGACCGTGCTGATTGG
sgRNA2 sequence	TTTCCTAGACACAATGTATAAGG

 

left_200bp	right_200bp
TCTTTTACAACGAAATCTAAGAAACCGGCATCGAAGTAAATATCTAACAGATTCTGGGGTAACCCCAAAAAAAAGCGTAACCTAAATCCTGAGATCAAGATAAAACTTAGCCGCAGGCCGAAAGCGCCAGTGCCTGGTTCGGTGACCCCCGATCGCGTAGTTGACCTTTGAAACCACCCACTAACCACCACCCACCAATC	ATAAGGTAAATTGGTTTGATTCCAAGAATGTTTTCCAAATAGTCTCCACTCGAAAGAACATGTTTTCCAAAAAGAGTTTGTTAAAGCGTTTCCAAGGGGTCAGATGTCAGAATTCAAATTCTGTTTGTTATACATTCAAAATGATTTGTGTTATACCACGCGTTATTGTAAGTTTGTCCCTATTTTTGTGTATTCTTAAA

 

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