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CRIMIC CR70873

Gene MED16

CG5465

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:22183138..22185892	58C5-58C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAATTGAACGAGGGACAGTG	GGTTCTCTAGGAATTTCGACTTCTGG

Images

Construct Design Sequences

sgRNA sequence	AGAGTAGTGTTGGCAGACGCCGG
sgRNA2 sequence	TTAACCGTATCCATGAATTGTGG

 

left_200bp	right_200bp
AAATGATTAATGATTTAAAGCTGCTGCGCTGTAAAAGCTTTATTTGCTGTTCAGAATTAAAATATTAATGAGCACTATCTTAAATCTAAACATAAAAACGAAACATTTTACAGTAACAATGGAATCAAAAATGCAGTGGAAAAACGCTAAACACCCGCGAAACAGTGATGTAAACTTCTTCACAGAGTAGTGTTGGCAGA	TTGTGGTTGGTGTATTTGCTAACGGTTTGATATTAACAACAACAACATATCGAAGAACTTGCATCACTAGCATGTCAATCGATTTCAGGACGTCCAATGTGTCTGTCGGCTGTCGGCAATCGATATCTCCTCTGCAAATAGATATTTTCAGAATAATTGGCTTATTTCACTTCGCCCCAACGCTGCAGTAATGGTAAGTG

 

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