CR70874

CRIMIC CR70874

Gene CG1239

CG1239

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5733513..5734702	83C1-83C1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CAACTTCTTAGGTATGGAAGTCCC	GAAACTTTTGTGGGCAGACAGTG

Images

Construct Design Sequences

sgRNA sequence	TTATACTTTGGATTTCACAATGG
sgRNA2 sequence	GACTTTGTAAAAATTTGGATCGG

left_200bp	right_200bp
GGAAAGTGGTATTTAAAACGGTCACTCTAAGTTAGAAATTACCGCAAGATGTGGTAAACATTTTAATTTGTATGTTTTTAAAGATATAAAAGGGCAAGGGCATAAACTCATTGATATATATGTAGTGAGTATTTAATGAGACATCAATAGTTCCTCAAGTCATTTTAACATACCAATGAATTCTTATACTTTGGATTTCA	CAAATTTTTACAAAGTCGTAGGAAATAAAAAGAAAATCAAGAAATGAAAATTATCTGAAAATTCAAGGGAAACCCATATATTTATTTACATCGATTACATCTAAATGTAATCAATACTACTTCATTCGCTTCACTTTACCAGGAGCGCTTGGTATCTTTTTCAGTACGTTGGGAATCCATTTCAGTAAATCGTCGTGTGT