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CRIMIC CR70877

Gene ND-20

CG9172

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:15985473..15986808	14A5-14A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CTACTTTTTTACTTGCCTTTTGGTCAGC	CTTTGTGTCCCGAAAACGTCAGGA

Images

Construct Design Sequences

sgRNA sequence	TCAACTTATTTCGATTTATAGGG
sgRNA2 sequence	TTGTTGTATACATTGTTTGTTGG

 

left_200bp	right_200bp
GACCTTCAAGTAAGTTGATTTTTTTAGCCAACCATTGCGTTCATGTGGGAAAAGGGCATAAAGTTCATGCTGCCGCAAGTGATGTGCTTGTGTGAGAATAGGATATCTAAAGTAAATTCGTATTTTATAGTATATTTTCCTGGTGCATTTGGTATTTTAGTTAGTAGTTCGGCGGCGGTCACATCATACGCATCCCCTATAAATCGAAATAAGTTGAAGTTACATAAATAGGTATTAAATGTTTAAATGCCCAGTCCTAGTATAACTACAGATTTACTTTAGCAGGATCAA	TGTTGGATCACGAGGATATTGCAAATAAATGCCTTTCGATATAAATACAAACATTCAAGCTGTGCTTCTGTATCCAAACGTCAGCTTGAATCGTACGTGTGTTTGCCAACGGTCGTAATGAAAATCGTGAATTCGAAAAAAGTTTTGTTTCCCTTCAAAATGAGCGTGAAGTTAACGGCTCCTTCCTCTTTTTTTTATC

 

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