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CRIMIC CR70879

Gene Nf-YA

CG3891

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:9440056..9442367	67B6-67B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTCACGACAAGCCTCTCG	ACAAACAATTTTTAACAGCTGGCTAAC

Images

Construct Design Sequences

sgRNA sequence	GGGGGCTTGAGGTTGAGACACGG
sgRNA2 sequence	AATAGTCGCCTCCTAGCTGTAGG

 

left_200bp	right_200bp
GCCAAGAAAGTGGTAAGTTGGCCAAAGAAGAGAGAACAGCGCAAGATCGGTATCGTTAGTCATTTGGAGGCATAGATCTAGTTGTAAGGTTAGATTTGTGCCATAGTGCCTAAGAACGAATCTTTGACGTGCCATCCACCCTCAAAATCGGCTTCATTCACTTTTCCAGTAAGTGTGTGTGTTGGGGGCTTGAGGTTGAG	TGTAGGATACAAACTAGATAAGATTGTAGTCATAGCGTTTAGTCCTAAGCCAGTCGCTCTGTGTCCATTTGTCCTCAATCACACCACTTTAGTCGGCTCACATAGTTTTCATTTTTTTTTTTTTTCAAAATTAAGTCTTCATATATTATTTTCAAAGGGAATTTTTGGGTTTTTCTATTTATTTTCATTCACTTAGATTG

 

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