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CRIMIC CR70880

Gene Nup160

CG4738

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:11123777..11129335	32D1-32D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGTGCAATGCAGTGTGAC	GGTTAACAATTGTGATATGTCCATCAAGG

Images

Construct Design Sequences

sgRNA sequence	ACTACTGAGCGTGTTTACGCCGG
sgRNA2 sequence	GCAGATGCGAACGGATCTTCAGG

 

left_200bp	right_200bp
GATTTAAAGGCATCTTATATCATTCCTGCTTTGCTATAATAAACTTTATTGTCATGAAATTACATTTAGGAAAATGTACAATTTTTAACTAACTTCTGTAATTAACTTTTGTATGTGAGTATAAAAAGCTATGTCGACTATTTTGCCTGGTGTGCACCGCTGGCGTAAGCGTTTTCCAACACTACTACTGAGCGTGTTTA	TTCAGGAAGAACGTTAATAGTTCGTTTAAATCAGTTTATTTAATAAGTTTTTTGAATTAAATGCTAAAGCGTGTAAATCGTTTAAAAATCATCCGTCATGTCCAGCTCAGAAAGGAACTTTTTGTAGATGTTCATAAACTGAGCGACGAACGCTTCAAGATGAAAAATGTGCTTGGCGCCGGACTGCATCCGGTGCTCGT

 

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