CR70882

CRIMIC CR70882

Gene Bace

CG13095

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8494893..8496307	29D2-29D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAATCATAACAAAGTCCAGCTGC	GATAACCACTTCAGTCTCCTTAAGTGT

Images

Construct Design Sequences

sgRNA sequence	GATGGTCTTGAACATTCTCTTGG
sgRNA2 sequence	CTTGAGGTGACCCAGACATTTGG

left_200bp	right_200bp
CTCACACCTGAAATGATAATATGCCAGATTATAAATATCGAATGCCGGACGAGCATTAAGAACTCATACTGCTTAAGACTGGAGTGGTCAACAAATCTTATCACCGGACAATTTGGGCTGTGATTGATGGCAAGTGCTATAAATTAGACCCGCTACACCGATCGTCAGTTAGTCGAACTCGAGTAGACTAGAAACCAAGA	GTCTGGGTCACCTCAAGTGGGTCACCTTTCGAAGGCTGAAGGTGTAACAATCTAATCTGTTTACAACTTTTTAAGTGGATAGGTTGTGAGTAAGCGACTCCAATTGCGTTTTACAGTCATGGCTATTTATGCTTATCTGCATTTCATATAATAAATGAATTACACATTGGACTTTCTGTAAATTGTTCGAGAATGTATAA