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CRIMIC CR70884

Gene noc

CG4491

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:14490859..14494019	35B2-35B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTGCCGACGCATTTCTGTG	CAAGTCTGTGCGAGCAGAGC

Images

Construct Design Sequences

sgRNA sequence	TGGGATGGCCGTAAAATCTATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTAGCCTTGTCTCGCAACTCACGTGGATTACCCTCGAGAAATCACACTAAGCAACTGATATGGTTGTCTTGGAAGGAGGCGGCGGCGTTGTTACCATCGGTAACAACCAGTACCTTCAACCGGATTATCTGGCCCCATTGCCAACGACGGTTAGTATTGACTAAGTTAAAAGCTGTAAAATTTTGGGATGGCCGTAAAAT	CTATGGTTTTAAATTTTGCGTACTTAAGAATTAAATTTGGAAAGTTCATAACATCAAACAATTTTTCAAATCTAAAAATTTATATCGTCATTAATACTTTCACGAACAAACTTTCTTTGGAAGAAATTGCATTTTCTCATAGTTTTAACTTCTCACCAAATTAAAATCAATATTATCTTAAAACTCGTAAAAAACTTTAA

 

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