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CRIMIC CR70885

Gene PIG-S

CG44255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:24913216..24917864			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAATACGTGTCGCGCAACAC	CCTGCACAGCCGTTTTGATC

Images

Construct Design Sequences

sgRNA sequence	TCGCGGTCGTAAGCGTTCTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGACCTGTTTTTGTTATTTCGTGCAGCTACAAGGAGTAGCATGTGATCCTGTTTCACAACATGCGCACAATTACTCGTCAAGGACTATTAACATTCATACCGTTCTCCTGTACAGAAACTAATTTACATAATAAACTTTATAAAACACAAATCAAAATATTATAGTTTTTTGTCGAAGTAATAAAGTGTGAACCCAAAG	AACGCTTACGACCGCGATAAATCAGGAACTAATCAAACACTTCTCTTTAACTTTACAGATAAGTTCCGGATCGGGGCTACACTAGCATTTATCGTGGTCATCATTGGAATTGGCGTGCCCATGTGGTGGCGCACCACCACAGTCTACAGGTGAGTCAACCATGTCTGGCGGCGTTTTTCCAATTATACCTAAATGTTGCA

 

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