CR70888

CRIMIC CR70888

Gene CG6659

CG6659

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:18653392..18657286	17C7-17D1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTCGCGAAGAGTACTTCCC	CTCAATTAAGCGTTCAAGTGCCC

Images

Construct Design Sequences

sgRNA sequence	GATATGGATGCCAATGAGAGCGG
sgRNA2 sequence

left_200bp	right_200bp
GACAGCATGCTCGAGTGGATCAAGCTGAACACAGAGCGAGATGCGGTCTTTGCCGGCCCCGTGGACATCATCGGCACCGTGCACTTGACCACCAAACGGCCCATAGTCAACCATGCGCACCTGGAGATGCGTCAGATAGCGTAAGTTCTCCCAATCTGAGCGAGATAGCCTATCGACTATGCCCCTGCTCTGTCCCGCTC	TCATTGGCATCCATATCCTGGCCATGCACGTACTGAAACTTCCGCCTTTGTTTATCTTTTTTAAGGGAGCGCACGGAGCACGTGTACTCGGTGTACAGTCGTCAGCAGTCGTCGGATATCTACAACCAGTGCGCCCAGCTGAAGATCCAGTACTTGATCATATCGCTGGACGAGTGCACCAACGAAGTGCGGTGAGTAGA