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CRIMIC CR70893

Gene Sgp

CG13091

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:8519690..8521809	29D4-29D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACAGGATTTCTAGGAAAAGGTAAAGC	GCTTCCAAGCCTTTCATCTCCTTG

Images

Construct Design Sequences

sgRNA sequence	ACGAATATGGAAGTCACTATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATAAATTCAAAACTAAAATATGTTAAAATTCAAATTTCAGTGATCATTGAAAAGCTCCTCCGTTCAACGGATGTAAGGCGAGTTTATCTATTAGTAAGGCCAAAAAAGAATGAAACGGTGGAAGGACGGTTTCAGGCCTGGAAAGATGAGCCGGTGAGTCCAAGAAGATGATGATAAGGATGTGGGTGCCCATCCTATA	GTGACTTCCATATTCGTATGATCTTAATTCGGGTTGAATGCTGATATTTTTCAGGTTTTTAAAATCCTACTCAAGGCTAAACCGGAGGCTCTGAAGTTGGTGACACCCATTTCGGGAGACTGCAGCGAAACCGGTCTGGGTCTCAGCGACGGGGATCGCAGGATCGTGACCGCTGATGTGCAGGTGATCATCCACAGTGC

 

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