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CRIMIC CR70897

Gene Mec2_new

CG31030

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:19188035..19190193	18A6-18A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGCTTTATCAAAGTAGTACTAGTAGC	GAAGATCAGCACGGAGAACAGG

Images

Construct Design Sequences

sgRNA sequence	TAAGTAAAACAGGACTATGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATAATCGTCAATTGCTACAACATTCTAATTGTATTGGCGCATATATATTACACCTGTCTATATTTTTAATCGTAGGAATTTTTATTGGACTCCAAGATAAGCGGGCTGATTGGTGTTTATCCGTGTTTTCTTTTTTTTGATTGTGATGGCTATTGCTTTTCACAGCTTTCTCTTTTTCAAGTTCTTACATAGTCCATCA	TAGTCCTGTTTTACTTAATGATAAGTTCGTATTTCCGGAATCCGGACAAAGAAGGTACTTCATCTTGGCCATCGAATTGAAGTGGCCTTATCTACGTTTCTCTTGCGGCACAATTATTGTACATATATGGCTGAAATATGGCCAGTTTGTAGATGAATATTTCATATTCATATTCCTAACATCCCATTGCAGCGGAGAAC

 

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