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CRIMIC CR70901

Gene Grip91_new1

CG10988

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:13736570..13744683	12B4-12B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAAGTGTGTTGCATAGTCGTAAATGTTC	ACGTGAGTTTCCTGTACAGCTC

Images

Construct Design Sequences

sgRNA sequence	TAGACACTATACATAATTGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACAATTCAAAGTATTCAAAGATATTTATCCGAAAGTAGATAGTTTTGAATTGTTTTTAAATGACTATTGATCACATATACTTAACTATAAAAGTATGAGCAAGTTTCTTAGATCCTCAAGGTCCTCAATACGTACAATTTCCAAATCAATGAATCTAAGGATACGTAATGCAACACATACACTAGACACTATACATAAT	TGTAGGCTTCATTGCCCTTGTTTTAATGCACTTTGTTCAATCTTTTGCAGAAAAAGCAATGGATCATTTAAGCAAAATGATTGCCGCCAATAGTCGGGTCATTCGGGACTCAAATATGTTGACTGAGCGCGAATGTGTCCAGAAGATAATGAAACTGCTGAGCGCCCGGAATAAGAAGGAGGAGGGCAAAACTGTGTCGG

 

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