CR70902

CRIMIC CR70902

Gene VhaAC45RP

CG31030

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:30164692..30166419	99E1-99E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTAAACTAAAGATCCGTTACAGAAACACG	GGTTATGAGATGTACCCGAAAAGCATG

Images

Construct Design Sequences

sgRNA sequence	AGCTGCATTCTGGAGTCGGATGG
sgRNA2 sequence	TGTTGTACGTGTATGTGCTATGG

left_200bp	right_200bp
TGGATTCACGTTCCCTACATAACTCAGAAGAACTGACCTACTATATGAGAACTCGAGCATAATCCACCACCCATCGAGACCCACCACTTCTGGTTTATTTCTGCGCATGAGTCTTGCCCAATCGATTTTTCACTACATACATACATCCCCCATTCAATCGAGGCCAGGCAGTCTTTGGTGATAGACGCACATTTCCATCC	CTATGGTACTACTGAGTAATCCAAAGTGGAGATGAAGAAATATAACATATATCAGAAGAGAAAAATAATGCAGTTTTTCTGAAATAGTACTTGTTATGAACAAGTGTTTAACTGCCTGTCATGTACTTCAAAATTCAGATAATTTCCTCCAATTGAGTTAAAATTATCAATATTAATCGAAGCGAAATAAACTAAAATAC