CR70905

CRIMIC CR70905

Gene CG13097

CG13097

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8512174..8514868	29D3-29D4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAATCCATCTTCTAGTTGGGC	AGTGGCTACGTTACTTGCTTTG

Images

Construct Design Sequences

sgRNA sequence	CTGTTCCATCTCTCACCAACTGG
sgRNA2 sequence	TGCAACAAGGATTGCTATGTGGG

left_200bp	right_200bp
TATACGGATTCTGGTCTACTGTGTAAGCAATTATGATTTTTTTTAGAGCTTTGTTTTCTTCAAGTTAAATATATTTATTATTTTTGGAATAACAAATTTTGGCTCAAAACAGGATTCCAACTCGCATAAAGTTTTTAAGCTTGGAAAAACTTGTACTTGTATCACGCGCTCATCTCTATGTATCTGTTCCATCTCTCACC	TAGCAATCCTTGTTGCAAGAATATCTTTAGCATGTACTGTGTGTCTGGGGAGTCCCCAATTTGGTGCTCCATGGGAGATGCCATGTGTCGGTACTTTTAATGTTCCATGTGCGCGGATCCATCGTATGTGTATGTGATGCGTGGGCGCTTTGTGTGTGGCGGCGTGTGAGTCAAGGACATTCTGTGTCCTGTTGAGGCGA