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CRIMIC CR70907

Gene Npc2c

CG3934

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:10060252..10061136	85F8-85F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGACAGCACGCCAATTAGAC	GAGTCCAGCAGACGAACTTCTAG

Images

Construct Design Sequences

sgRNA sequence	CATAGTTCAGATCTATAAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGAAACCACTTAATATCATTAACTAATTTCTGGTAAATAAATATTCCAGGTGCCGACAGCAACTATCCTCAGCCACTGATGGTGCAAATCGACGATTGTGACGCATTGCCCTGCGATTTGTGGAAGGGAACCGAGGCCAAAATCGACATCCAATTTGTTGCCAGTAAGTAAAATGCCATGGTGCATAGTTCAGATCTATA	AATCGGAATATTATTTTGATACTATTTTAATTTCTACTACAATATTTTAATAATATTTTTGTTCTCCCTTCCATTAGCTCGCAATACCATGAAGAAGCTATCAGCCGAAGTGCATCTGACCTCGCTGGGAGTGACCATACCCTATGACCTAGAAGCCTCCCGTGGCAATGTGTGCAGCAATCTGCTCCATGGCGCCTACT

 

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