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CRIMIC CR70910

Gene Plp

CG33957

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:15066081..15094724	71B2-71B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTTCAGAATGGCACTCTGAACAGTC	GCATGTGGAAAGGAAACAGGG

Images

Construct Design Sequences

sgRNA sequence	TTCTCAGTGCGACAATTGCGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTATATCGACAATTTTGAATCGCAAACTAAATATTCGCCAGATATATAATTATATATAATTTTCAGTAAAAATAAATATATTATTTTGAATGCAAACTGCGGAAGTTTCGAAAATGTTTCAGGGTCCGAAGATCCCGCTGCGAGTGAGTTTTTCAAATGGAGCTTTTAATATCCGATCAAGGTTTCTCAGTGCGACAATT	GCGCGGTGTGCGGCAGCGAATGTTTATTTATTTTGATGAATTCCGCCGAAGGCAATCATAAACTATAAAAATATATAATTCAATTTAATTTAATTTGTTGTGCCGTCGATTGTCATAATGTTTTCCTCATGCATTCGTTGACGGACATTGTGCAATTTTTCTGCTTGAGATATGTGATTTATGGCAGAATTTTTTTTTTT

 

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