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CRIMIC CR70913

Gene Cftr

CG5789

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3R:24536204..24543914	96A6-96A7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGATCTAGACGAGAGCTATCCATC	GTGCAAGAACAGCTGGAATTAAGG

Images

Construct Design Sequences

sgRNA sequence	TTAACTGACAACAAACACTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAATCCGCGACAGCATGCAAATATTATTTCACAACTAATATTTGCCTGGGCGATACCATTACTATATCGAGGTTCCCGCAGAGGCCTTAATACGGATGACCTGACACAATGTCTGAAGGAAGATCGATCCGAGCAGTTGGGCGACCGTTTGGAGGAGTAAGTACTGAGATCTAACATCCTCTTTAACTGACAACAAACA	CTAAGGAAGTTTGACTTGCTACAAAGTCGTATTAAACATTTGTCAATTGTGATCAATTTTTAGTCAATGGTTTAAGGAACTAGAGCGATCTCACCGCAAGAAACAGAAGCCGCATCTCAGAAACGCACTTTTCCGCTGTTTCCTAGGACCCACGATCATCAATGGCCTCATTTGCTTTATCTACATAGTGATCAAGTGAG

 

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