CR70918

CRIMIC CR70918

Gene CIA30

CG7598

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:29736965..29738070	99B9-99B9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAATGACAACAAAACCGGG	GCGAAGAGAAAGGTTCTGCAAAAG

Images

Construct Design Sequences

sgRNA sequence	TCGCAGGAGGCTATTCATCGTGG
sgRNA2 sequence	AACACAAACGTAACGTTAGGCGG

left_200bp	right_200bp
ATTGTAGCATCAAGTGGGCAAAGTATGTAAGTTTGATTTTCAATATTACATTTCAACTGTGCTTATCTTTTATCGAATATCTACTGTGCACAGCTGTTTCGCTGAGAATGGCAACTGGACCAGCTGATTGTTGTTACGTTGTTTTGCTGCACAGGCTGCTAGAGAAATAAGATTTACTTCAACCTAAACCAATGCCACGA	AACGTTACGTTTGTGTTATTTAATTGTAGAAACGTACGAAGAGCAGTAAATATTGGTTAACAGAAACATGCACATTGCCTTTACATTTGGGAAATTCAATATTTATTAATATATATAAATAGTAAATTCACGAATTAATATTTTTAGACCATCACTCATAGTCAGTTACAACTGTACCCTCAGCTGGTTATTGCTCTCTT