CR70919

CRIMIC CR70919

Gene ECSIT

CG10610

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:5856585..5857914	83C5-83C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGATTCGTGAAGGTAGTCACAATGAG	TGTACACTTAGTTTCGGGTTCTGTG

Images

Construct Design Sequences

sgRNA sequence	ACAAAACATTGCACAAGCTGCGG
sgRNA2 sequence	TTTGTATACCATAGAATTGATGG

left_200bp	right_200bp
CTTTTGTTACTAGCGTATGACTTTAAGCGTGTATTACGGTCATGACACTATTTAAGCTTGAAAATCTTTGGGTACAGCCGAAACTAAATACAATTTTTCGCCCTAGAATTTACCAAATTTGGCTAAATACGCTAGACTATCGGCACTATTAAAACATATGTTGCAGCAGCAGTGCTGCAAAACGCGAACTAGTTCCGCAG	TGATGGTAAATGTTGCTTAAAGTTAGTCGGTTTTGATCATATGTACATATATATGTACATCTGCTAGAAGGAAAAAAATTAATACTTCGGTAATTATTTATACTCTTTGGCTCAGACGCGAATTGAAAGCAACATTGTATGCATTTTGGGGTGGATTTTAATGAAATAAGGTTAATTGAGAAGGATTACTCACAACATCC