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CRIMIC CR70920

Gene Mics1

CG1287

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:7500951..7502376	84D9-84D9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTTAAACTGGTTCAAAGCGG	GCAGTACAGTGCTTTAGCAGGG

Images

Construct Design Sequences

sgRNA sequence	TATCTTGTACAACGTCAGAAAGG
sgRNA2 sequence	ATTTGTGGTAAAACCCCAGGCGG

 

left_200bp	right_200bp
CAACTGATAAACTTATGATGATGATAAAATAATAATTGCAGAAAGCAATAAATGAACACATTCATTTTAGATGTAACCAAGCTGCCCTTTCAAAATCAATCTGCTATGTAGCGTTGGTTAGTCTTGTCTCAAAATTTGTTTAGCTAAATTGTTCATTAAAACTGCCAAAACTCATAACCCCCTTCTTATTATTACCTTTC	GGGGTTTTACCACAAATTGTATAATTTAGTTAGAATTACCCATTTTCATATTTTGAAATGTATCTTTTCGAAATTGTTCGAAGCTAAAGCACAAATCTAAAAAATAACATTAAATTTGAAATGGTTTTTCAAATTTCTAAAAACAAGAGAGTACGAAACAAATATAAATTTATGAACAGGCAACAAAATTGTGTATTTAG

 

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