CR70921

CRIMIC CR70921

Gene ND-B22

CG9306

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:13370103..13371110	34B8-34B8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTGGTTATAGGTATCTACTCTAGACAGG	TGTTACTGCTAGTTCATGAATCACGG

Images

Construct Design Sequences

sgRNA sequence	TGTTGTTGTTGCCTCGGCTGCGG
sgRNA2 sequence	GATTGGATGTGATTTGATTACGG

left_200bp	right_200bp
AACAAAAGCTTGTATAAAATGCAAAGATAGCCATTGCTTTTGTATAGAAAATTTAAATCAAATATATAAATATTTTTTGAATTTCCATTCATTTCAGAGTTGTCGTTCTCTAAAATTATTTTTCGCTTCGCAACACATATTTTCAGGCGCAGGGCTTTAGAGCACTGGACTTTTGACAGCTGTTGTTGTTGTTGCCTCGG	TCAAATCACATCCAATCTTTGTCTTTAAAAGTAAATCTATTGGAACTCACATTATGTGCTATTATAAATGTGTTGTACTGGAATTCAGTCGATCTTCCTTCTGCCAAAATAATTCATTGCCAATTTACAATAATAGTGTATACAATTGGTTATGCAGATCAATTCCCAGATTATAAACGCATTACATTTTAGGTTACAAA