CR70922

CRIMIC CR70922

Gene Ndfip

CG32177

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:17647442..17654280	74E4-74E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATAGTTTGCACAAAATTCACCATG	GACTTGCGAGATATCCCAATAAGTTTGT

Images

Construct Design Sequences

sgRNA sequence	TGGTTGTTGTGCGGCATGACTGG
sgRNA2 sequence	TCTTGTAAATACGTTGTGGGTGG

left_200bp	right_200bp
TGCATGTAAATACTAAACAGTTGATCTGTTAAGCTACACGAAAATAAGGATATCTTAAATTTAGTATGTAAGAAAGAAAAGAATTTCTTAAGAATCTTCTTTTCTGAGACGTCACCTTAGGCTGTCAAAAAAATTCATATGCTTTGTAACCCCTTAAGCACTGAATTAATTTTCCCGTTTTCCGTTTGTAATTTCCAGTC	GGGTGGCTAGGAGGCTCGAATGCTACCAACCAACCGATCCCAACACCCTCTATTCCCCAGACCCAGCACACGAAAAATCAACGCGAACTTATTTTGCAGAGTTCTCGAAATCACTATGGACTTGAAAGTATTCCCTCAAATGTATTAGTCAATCAATTTCTTTAAAATAGAAGTAATGTTATTCAAAAATATACTTTGTA