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CRIMIC CR70924

Gene p24-1

CG1967

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:11850909..11852530	10F1-10F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCAGTAGCCGATAGCATATACG	GCCACGCCATCTGAAATGGAAT

Images

Construct Design Sequences

sgRNA sequence	GAATAACAGGCGGTGAAGCAGGG
sgRNA2 sequence	AATATTACATGTGTCGTTTAGGG

 

left_200bp	right_200bp
TATCGTGCGGCGAATAACACAAAACGCCGAATGCTGGCGATATCGATAGCATATCACAAATAACAAAAACACGTCACGTAATCCGGACGGAGGCTGGTAAGTAAATGGAATTGGAATGACAGGTGGCATAGCATTTGGCCGGAATCTTGGCACTGACCGAGCGCATTTCTTTTGCAGCTGGAAGAATAACAGGCGGTGAA	ACGACACATGTAATATTTTTTGTTAAGACCCCTCCCGGTAAAATACATAAAAAGGCCAGAAAATACATTAAAAAAAAACCAGAACTCGATAGCTAAAGGTACTCACAAAGCGTTTCAATAGAAGGGCGTCAATGGGGCATGAATCTCGACCAGAAGCAAGGAAATCGAACAACGAATCGAAAACCCCTTTTGACTAGAGC

 

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