CR70928

CRIMIC CR70928

Gene pen-2

CG33198

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:18277439..18278762	55C2-55C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTAGATATTGGCAAATGAAACAGTGATC	GTATTTTAGAGATGGATAAAGCGCATTG

Images

Construct Design Sequences

sgRNA sequence	AAACACCCACATATTTGAAGAGG
sgRNA2 sequence	GTTATGTCTATGCACTGCCTAGG

left_200bp	right_200bp
CTCTGTTTCGATAACTTATCGATATCGATTGCGCTCCAATGGACTAAAAACTAAAACACAAATCAACAAAAGCAAAAATTGAAGTGAAAATATAAGCTATTACAATTTACATATTACATACAATTTACATATAAGTACGAAAAAACTTTCCTGGGCCGGAAACAGATTCGAAAACAAAGGAGCAAACACCCACATATTTG	CAGTGCATAGACATAACTAGATTAATTCGTTAGCAACTAATGATATTAAAAAAGACTTCATTCCTAAACAAAATTAACGTTTATTATTAATCCATCAGAAGTTCCCGTATATGACCCAAACAAAAGAAGTGCTGTTTTGATTTAATTACATATTTTTTATTCACATCTAAAGCTGTTTTTGTTTTTTATAATTTTCTGTA