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CRIMIC CR70929

Gene btsz

CG44012

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:14804090..14875488	88D4-88D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGCCACTGCATGTGACC	GGCTAATGATCTATGTATGAGAGCTG

Images

Construct Design Sequences

sgRNA sequence	GTAATGGGACTAATCTGTATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGAAGATCGTCAAAAGCAGGAACGGGTGACGGTGCAAGGTAGGCGGCAAGTGCTCCTTCGGCTTCGTCTGCCTGCCACCAAATGCATGCTCCTTGCACCGACGCCTCAAGCTGCTTCTCCCCACAGATATCTTCTCATAATGCACTAATGCAACTAGTTGTTCTAAGCTCCAGTGGAATAGCGTAATGGGACTAATCTG	TATGGGATTTATCTATAGATAGAAAAAAAAAACAGATAAACAGAACCCATGATCACACTTCATTGCACCTAAAAGTGCTACAAGCTCTGCAGAATTATCTTTCATATGTACAACTATGACATTAAAGTATAAAGTAAAGTGCCAGGGGGATTATTAATGGAGCTAAAAATTCCAAGAAATATTTTCCATCTATCTACTCT

 

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