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CRIMIC CR70933

Gene Rab3-GEF

CG5627

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:15089682..15113762	13A11-13A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACATTGCACACCACACATACAC	TCTTCGAGGGTATTCTTGCTCAC

Images

Construct Design Sequences

sgRNA sequence	GGAATCAAAAATCTGCGGAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTTAATATCAATTAGGTTACAGTACATTATACTCATTCCTCTAGACACTAATTCATTTAATGCTATTCATCGAAATAAACATAGAATATTTTGTTTAATTTAACACTCAAATGCATCCACAACACTTCATATCCAACTAGAACTGAGACAAGATCCCTCCTTGAAGCATCCTAACACCCACATCCTCACCACACCTTTC	CGCAGATTTTTGATTCCTCCATGGGATTCGTAGTGCCATTGTAGATTGCTTCAAACTTAAGCTTTGATTGTTAGCATCACTTAGGCGATTGTGTAGTCTTGGAATATCACCCAATTTAGGTCTTGATTACTAATACGAATTCGTTTTCGGATCCATAGTTTACGCTGCACGCAAAGAAGGCAGCTGGAGAGGCTTCCAAG

 

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