CR70940

CRIMIC CR70940

Gene rb

CG11427

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:4535635..4540626	4C7-4C7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTTTCCGATAGATTCGGCATCG	GAAGTTGTGTGTTTCCCATTTCAATAAG

Images

Construct Design Sequences

sgRNA sequence	TTTAGCTTTCTGTTTCCTAATGG
sgRNA2 sequence	TTAGCTATACACTTGTAAACGGG

left_200bp	right_200bp
AACTCATAAAAATTCAAAAGCAACATGAATGCCGCGAAATGTTTCGGTTAAAGTAATGGATTTCGTAACTAATACATTACTTTATTTATGTGCGTATCTATACGAAAAACTCTTGGTGCATTCCATCACTGTTAGTTAAAGTGTTCTAGTATTTTTGTGCTATGTGTTAATCGGCGACTATCGACAGTTGCCCACCATTA	AACGGGCGGGGAGAACGTAATCTTTGAAATTAGCTGATAACATTAGCTGAGATTACAGGTGTTTTAAATTCCATTTTCGAGAGATTATATGTAAATGTGCAGTGTAACAGATGCAACTTAAGTGTTCAAGTGGGATCGAAATCCAAAACTAATTTAAATTGCAATTTCAACTGTAATTATTACATATTCTTTAACTTGTT