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CRIMIC CR70948

Gene bip2

CG2009

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	4:459887..466828	102C1-102C		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCGATGAATTGTGGGACTG	GTCCAATAGTTCTTGAACATTGATGCTGAG

Images

Construct Design Sequences

sgRNA sequence	TATATCTTCCTTGTAACCACCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATTGGGGATTATGAAACCTCAATAGGCATGTAGATTGTATTTTTTGCAAATTATTATTTCATTAATTGTCTTGTAAACACCAAAAAAAAAAAAACAATAAATCTTTGATTTATATATATCTTAGATTAAGATATATCTTAGATTAAGATATATTTTAGATTAAGATATATCTTAGATTAAGATATATCTTCCTTGTAAC	CACCGGAACAGAAATAAAAATAAATAGTTGAGGAACTCGACTAAAGCGTTCTCTTATATTTTCTTTTTAATTTTGCTTGGTATGTTACTTAATAACGTTATTTATATGAGTTCAAAAAAATCTTATTAAAACCAAACAAAAAATCAAAAATGCTTTGTTTATTTGGATAAAATTTATAAAAATATTAATTGACAAGTATA

 

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