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CRIMIC CR70951

Gene drpr

CG5559

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:1715595..1731107	62B1-62B1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAATGGATTACTAATCTGCTCGC	ATGGTTAGCTGCGGTTACG

Images

Construct Design Sequences

sgRNA sequence	AGAGAGTGGAGTGTAGGTTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACAAGTTTATTATTAAGGTTTATATGTATATAGTTTATAGCCTCTGCAAGGGCATTCTATCTTTGGACTACCAAAGATCTGCACGTAGTGTCTTTGTAGCTATATTTCTGCTATATGTTATATGTTGCTTTGTAGCTTTTGTTTGTTTTTTTTTTTGTTTATGCCATCTTAACCCAAAATCTAACTCCGACCACCCACAA	CCTACACTCCACTCTCTCCCTCTCATGCACCCACGCAGTTCACTCCGGCTCAGTTATACAGGTGCAAGCAGGCTATCGGTCACTAATCCCCACCACCAAAACGGGAGCTTCCGGGAGCGGAACTTCTTCCGGCTCCTGGGTTTCCCGTGGCTAGCAAGCGAAAGGCAAAAGCAAGAAAAAAGCAACTAACTGTCGAACCC

 

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