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CRIMIC CR70953

Gene ClC-a

CG3365

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:11797244..11813207	86F8-86F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGCATCGAGATCGATCCG	ATTGAAGGGCAAGACTCAGGG

Images

Construct Design Sequences

sgRNA sequence	AGTAACCAAGTGAACTTAATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATCGAGGCGTTCTACTATGTGAGTGGCCTACGAGCCACCTGCCCATTTCATCCGCGTTGGACCAACGGCCATCGTCAACCCTTTTAAGCACTGGAAAAAAATAACCCTGAAGTAGTTCCATCATATCGAAAATAATCCATGACACGGTTCCCATTCCAAAAGTGAAGTGGAAAATAAGGGAAAGTAACCAAGTGAACTT	AATTGGCGATGGGAAAATCATAAAAAGCTAAATTTAAATAAATTAAGATTTTATTAAGGCCTAGTTGTCTGCAAAAATTGCTAAAAGAGAATCTTGATAGCGCATTAAATCCGCACATTATTACACAGTATTTATTTAGTTATTTATTTTGTTTTCAACGTCTGCAGCGACGTACCTCTTGCATTGACCCTCATAGCATA

 

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